Cigarettes
Comprehensive Testing for Cigarette Smoke and Filler
Overview
Our cigarette testing methods include the analysis of TNCO (tar, nicotine, carbon monoxide), the list of Hoffmann analytes, the abbreviated list of 20 FDA HPHCs, and the full list of FDA HPHCs (along with McKinney’s testing partners). We perform the ISO and Health Canada Intense (CI / CINT) smoking regimes:
- ISO Regime: 35mL/2sec/60sec – with 5 cigarettes per sample
- CINT Regime: 55mL/2sec/30sec – with 3 cigarettes per sample and vent holes covered
We also offer supporting services for cigarettes such as:
- In Vitro Toxicology
- Ignition Propensity (LIP / IP) for Fire Safe Cigarette (FSC) testing
- Filter Efficiency
- Physical Parameters
- Limited methods for whole cigarette and/or cigarette butt testing
Testing Details
Our combustible smoke and cigarette filler testing services fulfill the requirements of:
- Health Canada
- FDA (USA) 2012 Abbreviated HPHC Testing
- FDA (USA) Substantial Equivalence SE Testing
- Ignition Propensity (LIP) – ASTM Method E2187-09, ISO Standard 12863
Cigarette Assays
| FDA (SE) Substantial Equivalence Testing |
| Method | Description |
|---|---|
| AM-254 | Carbonyls by UHPLC with UV Detection: Acetaldehyde, Acrolein, Crotonaldehyde, Formaldehyde |
| AM-015 | Volatile Organic Compounds by GC/MS: Acrylonitrile, Benzene, 1,3-Butadiene, Isoprene, Toluene |
| AM-030 | PAAs-Primary Aromatic Amines (MS/SS) by GC/MS: 4-Aminobiphenyl, 1-Naphthylamine, 2-Naphthylamine |
| AM-011 | Ammonia in Mainstream Smoke by HPLC |
| AM-044 | Benzo[a]pyrene by GC/MS |
| AM-001 | Nicotine and Water in Mainstream Smoke by GC: Carbon monoxide, Nicotine |
| AM-020 | TSNAs-Nitrosamines in Mainstream Smoke By LC-MS/MS: NNK, NNN |
| Abbreviated List of HPHCs in Cigarette Filler (Substantial Equivalence) |
| Method | Description |
|---|---|
| AM-115 | Ammonia in Tobacco by HPLC |
| AM-052 | Metals in Tobacco by ICP/MS: Arsenic, Cadmium |
| AM-072 | Nicotine Content in Tobacco by GC-FID |
| AM-031 | TSNAs in Tobacco by LC/MS-MS: NNK, NNN |
| Health Canada Testing – Long List |
| Long List – Mainstream Smoke (ISO & CINT Regimes) |
| Method | Description |
|---|---|
| AM-001 / AM-007 | Puffs, Tar, Nicotine, Water, Carbon monoxide |
| AM-026 | Filter Efficiency |
| AM-006 | Pyridine, Quinoline |
| AM-015 | 1,3-Butadiene, Isoprene, Acrylonitrile, Benzene, Toluene, Styrene |
| AM-020 | NAB, NAT, NNN, NNK |
| AM-021 | Cadmium, Lead |
| AM-027 | Hydroquinone, Resorcinol, Catechol, Phenol, m&p-cresol, o-cresol |
| AM-030 | 1&2-Aminonaphthalene, 3&4-Aminobiphenyl |
| AM-036 | Mercury |
| AM-093 | pH |
| AM-111 | Hydrogen cyanide |
| AM-207 | Benzo[a]pyrene |
| AM-216 | NO, NOx |
| AM-254 | Formaldehyde, Acetaldehyde, Acetone, Acrolein, Propionaldehyde, Crotonaldehyde, Butyraldehyde |
| ENT304 | Ammonia |
| Long List – Sidestream Smoke |
| FDA (SE) Substantial Equivalence Testing |
| Method | Description |
|---|---|
| AM-005 | Formaldehyde, Acetaldehyde, Acetone, Acrolein, Propionaldehyde, Crotonaldehyde, Butyraldehyde |
| AM-006 | Pyridine, Quinoline |
| AM-007 | Carbon monoxide |
| AM-008 | Puffs, Tar, Nicotine, Water |
| AM-011 | Ammonia |
| AM-015 | 1,3-Butadiene, Isoprene, Acrylonitrile, Benzene, Toluene, Styrene |
| AM-030 | 1&2-Aminonaphthalene, 3&4-Aminobiphenyl |
| AM-041 | Mercury |
| AM-043 | Cadmium, Lead |
| AM-048 | Hydroquinone, Resorcinol, Catechol, Phenol, m&p-cresol, o-cresol |
| AM-111 | Hydrogen cyanide |
| AM-208 | Benzo[a]pyrene |
| AM-211 | NAB, NAT, NNN, NNK |
| AM-217 | NO, NOx |
| Long List – Cigarette Tobacco Filler |
| Method | Description |
|---|---|
| AM-115 | Ammonia |
| AM-031 | NAB, NAT, NNN, NNK |
| AM-052 | Arsenic, Cadmium, Chromium, Lead, Nickel, Selenium |
| AM-053 | pH |
| AM-089 | Sodium Propionate |
| AM-090 | Propylene glycol, Glycerol, Triethylene glycol |
| AM-095 | Mercury |
| AM-098 | Sorbic acid |
| AM-099 | Triacetin |
| AM-125 | Benzo[a]pyrene |
| AM-206 | Nicotine, Nornicotine, Anabasine, Myosmine, Anatabine |
| AM-218 | Nitrate |
| LIP & Toxicology |
| Method | Description |
|---|---|
| AM-050 | Ignition Propensity Certification |
| HC T-501 | TOX-001 Mutagenicity AMES Assay/ Bacterial Reverse Mutation Assay (Preincubation Method; GLP) HC T-501, 5 strains, 7 conc., 3 plates (single replicate) |
| HC T-502 | TOX-002 NRU Assay T-502 (Health Canada)HC T-502 8 conc, 8 dupl/conc, +/- control, GVP, PP, GVP+PP |
| LIP & Toxicology |
| Method | Description |
|---|---|
| HC T-503 | TOX-003 In Vitro Micronucleus Assay CHO-K1 (HC) – HC T-503 ST(+/-) act, LT( – ) act, 5 conc., 2 slides/conc, blanks, control, reference, single replicate)HC T-503 ST(+/-) act, LT( – ) act, 5 conc., 2 slides/conc, blanks, control, reference, single replicate |
| Health Canada Testing – Short List |
| Short List – Mainstream Smoke |
| Method | Description |
|---|---|
| AM-001 / AM-007 | Puffs, Tar, Nicotine, Water, Carbon monoxide |
| AM-015 | Benzene |
| AM-093 | pH |
| AM-111 | Hydrogen cyanide |
| AM-254 | Formaldehyde |
| Short List – Sidestream Smoke |
| Method | Description |
|---|---|
| AM-005 | Formaldehyde |
| AM-007 | Carbon monoxide |
| AM-008 | Puffs, Water, TPM, Tar, Nicotine |
| AM-015 | Benzene |
| AM-111 | Hydrogen cyanide |
| Short List – Cigarette Tobacco Filler |
| Method | Description |
|---|---|
| AM-031 | NAB, NAT, NNN, NNK |
| AM-052 | Arsenic, Cadmium, Chromium, Lead, Nickel, Selenium |
| AM-053 | pH |
| AM-095 | Mercury |
| AM-206 | Nicotine |
| Unique Testing Offered (for Larger-Scale Studies) |
| Method | Description |
|---|---|
| AM-044 | PAHs-Polycyclic Aromatic Hydrocarbons (MS) by GC/MS: Naphthalene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, Benz[a]anthracene (BaA), Chrysene, Benzo[a]pyrene (BaP), Benzo[e]pyrene (BeP), Indeno[1,2,3-cd]pyrene (IcdP) |
| AM-069 | Caffeic Acid in MS Smoke and Tobacco by LC-MS/MS |
| AM-069 | Caffeic Acid in MS Smoke and Tobacco by LC-MS/MS |
| AM-073 | Total Sugars by CFA in Tobacco: Fructose, Glucose , Sucrose |
| AM-074 | Reducing Sugars in Tobacco by CFA: Fructose, Glucose |
| AM-086 | Use of the Part-Filter Method for the Estimation of Smokers’ Exposure to Nicotine and Nicotine-Free Dry Particulate Matter (Tar)Nicotine in Filter |
| AM-086 | Solanesol in Filter (Butts) |
| FDA (SE) Substantial Equivalence Testing |
| Method | Description |
|---|---|
| AM-088 | Eugenol in Tobacco by HPLC with UV Detection |
| AM-098 | Determination of Sorbic Acid in Tobacco by HPLC |
| AM-120 | N-Nitrososarcosine (NSAR) by GC/MS |
| AM-127 | Acetamide and Acrylamide in Mainstream Smoke by GC-MS |
| AM-172 | N-Nitrosodiethanolamine in Tobacco by GC-TEA |
| AM-174 | Ethyl Carbamate (Urethane) in Tobacco Products by GC-MS |
| AM-175 | Ethyl Carbamate (Urethane) in MS Smoke by GC-MS |
| AM-177 | Aflatoxin B1 in Tobacco and Mainstream Tobacco Smoke by LC-MS/MS |
| AM-179 | Coumarin in Tobacco and MS Smoke by UHPLC-MS/MS |
| AM-180 | VOCs I in Smoke & Tobacco by GC/MS: 1,3-Butadiene, Ethylene oxide, Isoprene, Propylene oxide, Vinyl acetate, Vinyl chloride |
| AM-193 | VOCs II in Smoke & Tobacco by GC/MS: 2-Nitropropane, Acrylonitrile, Benzene, Benzo[b]furan, Ethylbenzene, Nitrobenzene, Nitromethane, Styrene, Toluene |
| AM-199 | Selected Primary Aromatic Amines (PAAs) in MS Smoke by GC-MS: o-Toluidine (2-methylaniline), 2,6-Dimethylaniline, o-Anisidine (2-methoxyaniline), 1-Aminonaphthalene (1-naphthylamine), 2-Aminonaphthalene (2-Naphthylamine), 3-Aminobiphenyl, 4-Aminobiphenyl |
| AM-212 | Select Sweeteners In Tobacco By UPLC-MS/MS: Sucralose, Saccharin, and Glycyrrhizic acid |
| AM-274 | Glycidol in Smoke by GC/MS |
| ENT183 | Flavor Components in Tobacco and Menthol in Whole Cigarette |
| ENT218 | Esters in Cigarette Smoke by GC/MS: Benzyl acetate, Ethyl acetate, Ethyl acetoacetate, Isoamyl acetate, Isobutyl acetate, Methyl acetate, N-butanol, Benzaldehyde, Cinnamaldehyde, Vanillin |
| Unique Testing Offered – Physical Parameters |
| Method | Description |
|---|---|
| AM-050 | Ignition Propensity Testing |
| AM-026 | Filter Efficiency |
| AM-009 | Physical Parameters: Total Weight, Filler Weight, Length, Tipping/Overwrap Length, Filter Length, Column Length (Rod Length), Vent Measurement, Total Resistance to Draw (RTD or Pressure Drop) (VO/VC), Plug Resistance to Draw (RTD), Ventilation, Circumference, Diameter, Volume, Density, Air Permeability |
| AM-188 | Cut-Width (of Tobacco Strands) |
| ENT520 | Particle Size Analysis via MOUDI Cascade Impactor |
Please reach out to our team for more information for your next validation project.